Abstract
Poly(lactic acid) (PLA) nanoparticles were prepared to be used as potential platform for vaccine systems. Commercial high molecular weight PLA (PLAHMW) with Mw 1.5 × 105 and low molecular weight poly(lactic acid) (PLALMW) with Mw 9.3 × 103 were obtained by direct polycondensation from D,L lactic acid and used to prepare nanoparticles by solvent displacement method. The effect of the molecular weight on the physicochemical properties of the polymers, on the nanoparticles dispersions and the amount of ovalbumin (OVA) adsorption was studied. The PLAHMW and PLALMW were characterized by Fourier transformed infrared spectroscopy (FTIR), thermogravimetrical analysis (TGA), modulated temperature differential scanning calorimetry (MTDSC) and gel permeation chromatography (GPC). The particle size distribution and ζ-potential of the obtained dispersions were measured by dynamic light scattering (DLS) and electroacoustic spectroscopy, respectively. The ovalbumin adsorption on nanoparticles was evaluated by the Bradford method. PLALMW dispersion showed lower ζ-potential values and larger sizes compared to PLAHMW dispersion. A minor OVA adsorption was achieved for PLALMW.
Highlights
Polymeric nanoparticles have revealed to be a promising way to delivery and transport many kinds of therapeutic agents in vaccination, as well as in the introduction of broad and potent immune responses, long lasting antibodies, cytotoxic T lymphocythes and mucosal immunity.[1,2,3,4] The nanoparticles may control and target the release and can be used to deliver or transport hydrophilicThe surface nature of the nanoparticles can be tailored for improving intracellular uptake and targeting
PLALMW looks as a white fine powder and PLA of high molecular weight (PLAHMW) as white fibrous material
This work showed the possibility of obtaining poly(lactic acid) of low molecular weight by direct polycondensation
Summary
Polymeric nanoparticles have revealed to be a promising way to delivery and transport many kinds of therapeutic agents in vaccination, as well as in the introduction of broad and potent immune responses, long lasting antibodies, cytotoxic T lymphocythes and mucosal immunity.[1,2,3,4] The nanoparticles may control and target the release and can be used to deliver or transport hydrophilicThe surface nature of the nanoparticles can be tailored for improving intracellular uptake and targeting. Several methods have been used for the synthesis of PLA including direct polycondensation, azeotropic condensation and ring opening polymerization.[11,22,23] PLA obtained by polymerization of lactic acid in presence of catalysts at reduced pressure presents low molecular weight.
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