Abstract
Abstract The retention behaviour of an extended set of flavonoid compounds on phenyl and cyano reversed-phase HPLC columns was studied and compared to behaviour on octadecyl column. The selectivity properties of methanol, acetonitrile and tetrahydrofuran as organic modifiers on each stationary phase are reported. Both the stationary and the mobile phases appeared to significantly change HPLC system selectivity. Specific stationary phase selectivity effects proved more pronounced with methanol: in particular the phenyl phase showed a greater selective retention for unsaturated flavonoids while octadecyl proved more selective for glycoside compounds.
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