Abstract
The effect of the method of immobilization of receptor layer on the analytical characteristics of label-free electrochemical immunosensors for determining antibodies to the measles virus is estimated. The receptor layer bearing the measles antigen was immobilized by dropping a model suspension on the surface of a glassy-carbon electrode, followed by drying in air and covalent crosslinking by electrografting of p-nitrophenyldiazonium chloride using glutaraldehyde as a crosslinking agent. The dependences of the peak current of the mediator system on the concentration of measles antibodies in model suspensions are linear and described by the equations I = –(28 ± 1)cat – (30 ± 4) and I = –(19 ± 2)cat – (15 ± 5) for the covalent immobilization and drop deposition of the receptor layer, respectively. Immobilization of the bioreceptor by covalent crosslinking does not lead to a significant improvement in the sensitivity of the determination; however, it expands the linear concentration range tenfold and decreases the total duration of the immunoassay procedure to 20 min with respect to the immunosensor with a drop-immobilized antigen.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
