Year-end Sale % OFF 
Abstract
The growth rate of the MAC16 tumour in cachectic animals was significantly enhanced by the hypolipidemic agent bezafibrate, while the growth rate of a histologically similar tumour, the MAC13, which grows without an effect on host body compartments was unaffected. Growth of the MAC16 in vitro was unaffected by bezafibrate, suggesting that it was an in vivo phenomenon only. The stimulatory effect of bezafibrate correlated with the maximum plasma levels of free fatty acids (FFA) arising from the catabolism of adipose tissue. Accumulation of 14C-lipid from 1-14C-triolein administered by intragastric intubation was enhanced in heart, gastrocnemius muscle and tumour of bezafibrate treated animals, while the total lipid absorption did not differ from solvent treated controls. The increased lipid accumulation in the heart, but not the tumour correlated with an increased tissue lipoprotein lipase level. The increased tumour level may arise from an increased uptake of FFA arising from a weakening of the bonds between FFA and albumin. These results suggest that growth of certain tumours is dependent on maintaining sufficient lipid levels and that the lipid mobilising effect of the tumour may be necessary to sustain tumour growth.
Highlights
Effect on the MAC13 tumour, which has a comparable histology and growth rate, but which has no effect on host lipid stores in order to investigate the relationship between mobilisation of lipids in cachexia and growth of the tumour
There was no difference in the growth rate of the tumour in the presence and absence of the arachis oil
Complexes of essential fatty acids such as linoleic acid with bovine serum albumin have been shown to stimulate the growth of human breast carcinoma cells in culture (Rose & Connolly, 1990) and to significantly enhance the growth of transplantable mammary adenocarcinomas in mice (Abraham & Hillyard, 1983)
Summary
Pure strain NMRI mice bred in our own colony were fed a rat and mouse breeding diet (Pilsbury, Birmingham, UK) and water ad libitum. Fragments of either the MAC16 or MAC13 tumour were implanted into the flank of male NMRI mice (starting weight 24-26 g) by means of a trocar, as described (Bibby et al, 1987). Animals bearing the MAC16 tumour develop weight loss 10-12 days following tumour transplantation and were used when weight loss averaged 2 g (average tumour weight 200 mg). Animals bearing the MAC 13 tumour were used when the tumour volume was comparable with that in animals bearing the MAC16 tumour. The dimensions of the tumours were measured daily by the use of calipers. The tumour volume was calculated from the formula: length x (width) divided by two
Sign-in/Register to view highlights & summary 
Published Version (
Free)
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
