Effect of the Host Plant on the Antioxidative Defence in the Midgut of Lymantria dispar L. Caterpillars of Different Population Origins

Abstract

The responses of gypsy moth larvae originating from two populations (oak forest, locust forest) to favorable (oak) and unfavorable (locust) host plants were monitored at the level of midgut antioxidative defence: the activities of superoxide dismutase (SOD), catalase (CAT), glutathione-S-transferase (GST), glutathione reductase (GR), glutathione peroxidase like (`GSH-Px like') and glutathione content (GSH). Short-term change of the diet (3 days) to locust leaves of the 5th instar larvae (oak population) provoked an increase in GST and `GSH-Px like' activities as well as in the amount of GSH. On the contrary, transferring the gypsy moth larvae (locust population) to oak leaves was followed by a decrease in GST, `GSH-Px like' activities, and in the amount of GSH. Feeding gypsy moth larvae from hatching on an unfavorable host plant such as locust, led to increases in GST and SOD activities and GSH content, as well as to a decrease in CAT activity in all instars studied (4th, 5th, 6th). The locust leaf diet caused changes in other components of antioxidative defence dependent on larval instar and population origin, a feature which could be ascribed to trophic adaptation of the gypsy moth to an unfavorable host plant.