Effect of the Glutamine-Analogue "Azaserine" on Embryonic Development of the Horseshoe Crab: (azaserine/inhibition of cell growth/morphogenesis/separation of embryonic area/horseshoe crab).

Abstract

Embryos of the horseshoe crab were treated with the glutamine-analogue "azaserine". This compound induced malformed embryos at the stage of enlargement of the germ disc. The embryonic areas of these monsters were separated into two pieces. This is the first report of monsters induced with azaserine. The effect of azaserine was counteracted by glutamine. Azaserine repressed cell growth of the embryos, and prevented development of their germ disc. During active morphogenic movement, the embryonic area of treated embryos was separated in the central region of elongation of the embryonic area.