Effect of the fluorescent tracer Fast Blue on the Pseudorabies virus infection

Abstract

Event Abstract Back to Event Effect of the fluorescent tracer Fast Blue on the Pseudorabies virus infection Gabor Marton1, Dora Tombacz2, Judit Toth2, Andrea Szabo1, Zsolt Boldogkoi2 and Antal Nógrádi1* 1 Department of Ophthalmology, University of Szeged, Hungary 2 Department of Medical Biology, University of Szeged, Hungary The Pseudorabies virus (Prv) inoculated into peripheral tissue infects neurons and spreads through synaptically linked cells. Retrograde fluorescent tracers can be used for the identification of the infected first-order neurons. The question can be raised whether these fluorescent tracers have an effect on the Prv infection. In this study we applied the fluorescent tracer Fast Blue to the cut sciatic nerve 24 or 48 hours after inoculation of Prv (Bartha strain) into hindlimb muscles of SD rats. The virus infection was studied also in the trigeminal tract: the Kaplan strain of the virus was inoculated into the cornea of SD rats and after 2 or 6 hours FB was applied into the cornea. In cultured mouse neuroblastoma cells the expression of different viral kinetic genes was investigated in the presence of FB. The application of the FB 24 hours after the PrV injection into the hindlimb muscles prevented the infection of lumbar motoneurons, while FB treatment applied 48 hours after virus inoculation was not able to inhibit infection of lumbar motoneurons. In the trigeminal nuclei the number of the virus infected neurons significantly decreased when FB was applied 2 or 6 hours after the inoculation of the Prv into the cornea. In the neuroblastoma cultures treated with FB the expression of the viral RNAs and amount of the viral DNAs was repressed. Our results show that Fast Blue delivered to the primarily infected rat neurons at an early stage of virus replication is able to prevent infection of motoneurons. The infection of the trigeminal nuclear neurons can also be effectively inhibited using FB. The molecular investigations confirmed our morphological findings. Conference: 12th Meeting of the Hungarian Neuroscience Society, Budapest, Hungary, 22 Jan - 24 Jan, 2009. Presentation Type: Poster Presentation Topic: Developmental neurobiology and subcortical functions Citation: Marton G, Tombacz D, Toth J, Szabo A, Boldogkoi Z and Nógrádi A (2009). Effect of the fluorescent tracer Fast Blue on the Pseudorabies virus infection. Front. Syst. Neurosci. Conference Abstract: 12th Meeting of the Hungarian Neuroscience Society. doi: 10.3389/conf.neuro.01.2009.04.065 Copyright: The abstracts in this collection have not been subject to any Frontiers peer review or checks, and are not endorsed by Frontiers. They are made available through the Frontiers publishing platform as a service to conference organizers and presenters. The copyright in the individual abstracts is owned by the author of each abstract or his/her employer unless otherwise stated. Each abstract, as well as the collection of abstracts, are published under a Creative Commons CC-BY 4.0 (attribution) licence (https://creativecommons.org/licenses/by/4.0/) and may thus be reproduced, translated, adapted and be the subject of derivative works provided the authors and Frontiers are attributed. For Frontiers’ terms and conditions please see https://www.frontiersin.org/legal/terms-and-conditions. Received: 02 Mar 2009; Published Online: 02 Mar 2009. * Correspondence: Antal Nógrádi, Department of Ophthalmology, University of Szeged, Szeged, Hungary, nogradi.antal@med.u-szeged.hu Login Required This action requires you to be registered with Frontiers and logged in. To register or login click here. Abstract Info Abstract The Authors in Frontiers Gabor Marton Dora Tombacz Judit Toth Andrea Szabo Zsolt Boldogkoi Antal Nógrádi Google Gabor Marton Dora Tombacz Judit Toth Andrea Szabo Zsolt Boldogkoi Antal Nógrádi Google Scholar Gabor Marton Dora Tombacz Judit Toth Andrea Szabo Zsolt Boldogkoi Antal Nógrádi PubMed Gabor Marton Dora Tombacz Judit Toth Andrea Szabo Zsolt Boldogkoi Antal Nógrádi Related Article in Frontiers Google Scholar PubMed Abstract Close Back to top Javascript is disabled. Please enable Javascript in your browser settings in order to see all the content on this page.