Abstract
A series of soy protein isolate (SPI)-based film-forming solutions were prepared by a co-drying process or by direct addition of alginate (ALG), with different degrees of phase separation and morphologies. For a non-phase separation system, a single Tg of the data was well fit by the calculation results of the Couchman–Karasz quotation; moreover, the initial state of SPI/ALG (20:1) and SPI/ALG (10:1) after homogenization and the state of SPI/ALG (20:1) after deposition for 30 min exhibited good uniformity; thus, a single Tg value was also measured in these cases (96.28 °C, 85.37 °C and 112.03 °C respectively). Two Tg values of 74.11 °C and 108.24 °C for SPI/ALG (20:1), 72.86 °C and 110.11 °C for SPI/ALG (10:1) were determined in the 100% of phase separation systems. The mixed interface, SPI-rich phase, and ALG-rich phase of SPI/ALG (10:1) co-blending system after deposition for 30 min were formed, resulting in three different Tg values of 74.32 °C, 90.15 °C and 125.17 °C. The SPI/ALG-CO (20:1) and SPI/ALG-CO (10:1) systems with co-drying process could significantly improve the interface properties and the surface activity of each component. The understanding of Tg led to new insights of defining the limit between the rubbery and glassy states in the film-forming co-blending systems.
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