Effect of the Electromagnetic Field (EMF) Radiation on Transcriptomic Profile of Pig Myometrium during the Peri-Implantation Period-An In Vitro Study.

Ewa Monika Drzewiecka,Jan Pawel Jastrzebski,Wiktoria Kozlowska,Agata Zmijewska,Anita Franczak,Pawel Jozef Wydorski,Lukasz Paukszto

doi:10.3390/ijms22147322

Ewa Monika Drzewiecka, Jan Pawel Jastrzebski + Show 5 more

Open Access

https://doi.org/10.3390/ijms22147322

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Abstract

The electromagnetic field (EMF) affects the physiological processes in mammals, but the molecular background of the observed alterations remains not well established. In this study was tested the effect of short duration (2 h) of the EMF treatment (50 Hz, 8 mT) on global transcriptomic alterations in the myometrium of pigs during the peri-implantation period using next-generation sequencing. As a result, the EMF treatment affected the expression of 215 transcript active regions (TARs), and among them, the assigned gene protein-coding biotype possessed 90 ones (differentially expressed genes, DEGs), categorized mostly to gene ontology terms connected with defense and immune responses, and secretion and export. Evaluated DEGs enrich the KEGG TNF signaling pathway, and regulation of IFNA signaling and interferon-alpha/beta signaling REACTOME pathways. There were evaluated 12 differentially expressed long non-coding RNAs (DE-lnc-RNAs) and 182 predicted single nucleotide variants (SNVs) substitutions within RNA editing sites. In conclusion, the EMF treatment in the myometrium collected during the peri-implantation period affects the expression of genes involved in defense and immune responses. The study also gives new insight into the mechanisms of the EMF action in the regulation of the transcriptomic profile through lnc-RNAs and SNVs.

Highlights

The electromagnetic field (EMF) term stands for the electromagnetic energy generated by geological structures in the Earth’s shell and by all the electric-powered devices
The overall statistics of RNA-seq data were constructed for six cDNA libraries
The EMF treatment affected the expression of 223 transcript active regions (TARs), and the majority of them, i.e., 65.47% was down-regulated

Summary

Introduction

The electromagnetic field (EMF) term stands for the electromagnetic energy generated by geological structures in the Earth’s shell and by all the electric-powered devices. In 1996, the World Health Organization started “The International EMF Project”, which aims to establish and assess the health and environmental effects of exposure to static and time-varying EMFs. The most common power frequencies of the EMF range from 50 to 60 Hz, which are classified as extremely low frequencies of the EMF (ELF-EMF) [5]. The number of ELF-EMF sources in everyday life is relatively high, as they are generated by the vast majority of household devices, lighting, and heating [1,3]. The consequences of the ELF-EMF exposition on the living organisms need to be elucidated

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