Effect of the degradation performance on carbon tetrachloride by anaerobic co-metabolism under different external energy sources

Abstract

In this research, a comprehensive study was carried out on the removal of carbon tetrachloride (CT) in the anaerobic co-metabolism (ACM) reactor. The experiments showed that when the hydraulic retention time (HRT) was 36 h, pH was 7, and influent CT was 2.5mg/L, the average removal efficiency reached 82.45 ± 2.56% in the glucose co-metabolism substrate reactor, exhibiting a dramatic excellent difference in reaction performance from the other two reactors (p < 0.05) and a favorable tolerance on the CT shock loading. The content of extracellular polymeric substances (EPS) and volatile fatty acids (VFA) demonstrated that glucose could supply more energy to protect the microorganisms, which was the appropriate external energy source. Moreover, microbial community structure and biostatistics analysis demonstrated that Pseudomonas was the most important dechlorination bacteria in ACM reactors, which might via dehalogenation process mediate the transformation of CT. The succession of methanogenic bacteria further demonstrated that CT degradation using co-digestion require to destroy hydrogenotrophic methane generation pathway and the external energy substances could make up the lack of hydrogen in the treatment of CT. The change of intermediate products hinted that anaerobic dechlorination process of CT in an ACM reactor was a sequential dechlorination process, and major transformation products measured were CF. Overall, this study has improved our understanding of the roles of CT degradation process in ACM reactors.