EFFECT OF THE COMBINATION OF ENZYMATIC PREPARATIONS ON THE AQUEOUS EXTRACTION YIELD OF THE OIL FROM THE PULP OF Euterpe oleracea FRUIT

E De S Ferreira,C A N P Herman,H L G Rogez

doi:10.1590/0104-6632.20180354s20170305

Abstract

ABSTRACT This work aimed to study the effect of the combination of four commercial enzymatic preparations (Celluclast 1.5 L, Viscozyme L, Ultrazym AFP, and Shearzyme 500 L) on the aqueous extraction yield of the oil from the pulp of Euterpe oleracea fruit. The enzymatic preparations were tested individually and in combinations of two, three and four, in triplicate. The negative control presented the lowest extraction yield (34.91%). The tests performed with the isolated enzymatic preparations presented an average increase in the extraction yield of approximately 30% compared to the negative control, confirming the important role of the enzymes in the extraction process. The tests performed with the combination of more than two enzymatic preparations increased the extraction yield by approximately 90% compared to the negative control, demonstrating the advantage of using combinations of enzymes. Among the different tests, those performed with the combination of three enzymatic preparations, including the cellulase class-type enzyme, Celluclast 1.5 L, stood out by presenting a high extraction yield (> 65%) and being statistically equal to the one using all four, which is interesting for the industry due to the reduced cost of the process.

Highlights

Over the last few decades, the population has been achieving higher longevity
The four tests performed with the isolated enzymatic preparations (EP) showed an average increase of the enzymatic aqueous extraction (EAE) yield of 28% compared to the negative control
Puangri et al (2005), Latif et al (2009), Gai et al (2013), Mai et al (2013) and Jiao et al (2014) showed that pectinase was among the main enzymes that contribute to a higher yield of the EAE process of the oil from papaya seed, sunflower, Isatis indigoga, gac fruit and pumpkin seed, respectively, shortly after cellulase, hemicellulase and/or protease

Summary

Introduction

Over the last few decades, the population has been achieving higher longevity Among other factors, this is due to better quality of life, with emphasis on the relationships between the health of people and a healthy diet (Aiello et al, 2016). The bioactive compounds present in food, especially those of vegetable origin, have been a frequent object of research due to their positive health implications (Liu, 2013). In this context, the fruit of the açai palm tree (Euterpe oleracea Martius), considered in recent years to be one of the new “superfruit”, are being extensively studied due to their nutritional characteristics that deliver positive health effects Regarding the pulp of E. oleracea fruit, research is divided into four categories: chemical characterization assays, in vitro assays, in vitro cell-based assays and in vivo studies using animal and/or human models

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