Abstract
Adult Musca domestica L. were chemosterilized by oral application of 5 μg/fly of P,P′ -bis(l-aziridinyl)-N-(3-methoxypropyl) phosphinothioic acid after they emerged. A considerable increase in the activity of acetylcholinesterase (AchE) during embryogenesis in the crude extract of the normal eggs was evident and was about double that present in the chemosterilized eggs. When the crude protein extract from chemosterilized eggs was purified by DEAE cellulose column chromatography, increased AchE activity was found. As shown by enzyme activity measurement and acrylamide gel electrophoresis patterns, AchE in ovaries, testes, and heads of chemosterilized flies appeared normal. During embryonic development period, the esterases showed a remarkable increase in the isoenzyme bands in the normal eggs over the chemosterilant eggs.
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