Abstract

Metagenomic analyses were carried out on municipal solid waste, with samples collected at two different depths (2 and 4 m) and distances (2 and 20 m) from a biogas extraction well. Tests were performed to investigate the hypothesis of oxygen entry in the waste mass through the cover layer. The results indicated the predominance of anaerobic microorganisms (37–51%), whereas the aerobic or aerobic and anaerobic facultative microorganisms presented values of 3–8%. Nevertheless, two-way Permanova analysis highlighted differences between samples with different distances and depths. The distance from the well was the most important variable influencing the community structures, and the most abundant genera were all anaerobic and included Methanobacterium, Methanosarcina, Methanofollis, Desulfosporosinus, Anaerolinea and Clostridium. The results strengthen the hypothesis of oxygen entry in the waste mass through the cover layer affecting the nature of the decomposition process, primarily for shallower depths.

Talk to us

Join us for a 30 min session where you can share your feedback and ask us any queries you have

Schedule a call

Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.