Abstract
The aims of the present study were to elucidate the regulatory effect of exogenous Tribbles homologue 3 (TRB3) expression on the Wnt/β-catenin signaling pathway and epithelial-mesenchymal transition (EMT) in transforming growth factor-β1 (TGF-β1)-induced mouse alveolar epithelial cells (MLE-12) and investigate the underlying regulatory mechanisms. TRB3 expression was upregulated and downregulated using gene overexpression and RNA interference techniques, respectively. TGF-β1-stimulated MLE-12 cells were examined for EMT and activation condition of the Wnt/β-catenin signaling pathway using Cell Counting Kit-8, flow cytometry, western blotting, reverse transcription-quantitative PCR, ELISA and immunofluorescence techniques. During TGF-β1-induced EMT, TRB3 expression was found to be significantly upregulated (P<0.05). In the TRB3 overexpression group, upregulated expression of β-catenin and EMT-related genes and proteins was observed (P<0.05), and an increase in fibrosis-related factors in the cell culture supernatant was detected (P<0.05); however, the results were the opposite in the TRB3 downregulated group (P<0.05). TRB3 may be involved in the regulation of EMT in TGF-β1-induced MLE-12 cells through the Wnt/β-catenin signaling pathway.
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