Effect of the administration of Lactobacillus paracasei subsp. paracasei NTU 101 on Peyer's patch-mediated mucosal immunity

Abstract

The role of lactic acid bacteria in gut mucosal immunity was investigated by comparing the enhanced effects in the Peyer's patches and spleen of BALB/c mice fed daily with Lactobacillus paracasei subsp. paracasei NTU 101 for 3 to 9 weeks. After feeding with Lactobacillus, the percentage of CD4 + T cells in both Peyer's patches and the spleen was significantly increased; however, expression of CD 154 molecules, which play a pivotal role in cell-to-cell communication, on CD4 + T cells and the percentage of B220 + B cells increased only in Peyer's patches. Compared with systemic serum IgA, Peyer's patch-derived immunomodulation induced higher levels of intestinal IgA +-producing cells in the lamina propria. Our data also showed that feeding with Lactobacillus induced stronger CD4 + T cell-dendritic cell interaction, enhanced CD4 + T cell and B cell proliferation, and increased IL-1β, IL-10, IL-12, IFN-γ, and TNF-α mRNA expression in Peyer's patches, but not in the spleen. Here, we demonstrate that following Lactobacillus treatment, Peyer's patches exhibited a more distinct capacity to induce CD4 + T cell-dendritic cell interactions, lymphocyte proliferation, and cytokine secretion than the spleen, and thereby promoted greater intestinal IgA production that could enhance immunosurveillance to prevent intestinal infections or other intestinal pathologies.