Abstract
Objective To investigate the effect of the adenovirus-mediated NK4 and MCP-1 gene co-expression in pancreatic cancer cell in vitro. Methods NK4 gene and MCP-1 gene were singly and conjointly transfected into pancreatic cancer cells SW1990 by adenovirus vector. The expressions of NK4 and MCP-1 were detected using RT-PCR and ELISA. Then the influence on the growth of pancreatic cancer cells and vascular endothelial cells were observed. Results When MOI was 60 efu/cell, the infection efficiency of SW1990 was 100%. The expression of MCP-1 and NK4 mRNA were the highest in the 5th days in all the three groups. The expressions of MCP-1 and NK4 in conjoint group were lower than either single MCP-1 or single NK4 group. There was no significant difference in the growth curve of all the three and control groups. Both AD-NK4 and conjoint groups can inhibit the growth of vascular endothelial cells, but no significant difference was found between the two groups. Conclusions Adenovirus-mediated NK4 and MCP-1 genes can effectively infect SW1990, and did not show inhibiting effect in vitro. However, NK4 may play the role of tumor suppressor through inhibiting tumor angiogenesis. Key words: Pancreatic neoplasms; Heptatocyte growth factor; Monocyte chemoattractant protein 1; Genetic therapy; Adenovirus
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