Abstract
The present study is to investigate the effect of the active fraction of Flos Daturae (AF-FD) (dried flower of Datura metel L.) on IFN-� induced apoptosis in a human keratinocyte cell line (HaCAT), which is a widely accepted mimic model of psoriasis. Accordingly, we used MTT and LDH to assay cell viability, FCM and Hoechst 33342 staining to assay cell apoptosis, and Western blotting to assay the expression of keratin 17 (K17). Administration of IFN-� (5-125 U/mL) decreased MTT reduction and increased LDH leakage in a dose-dependent manner. Twenty-five U/mL IFN-� ele- vated apoptosis significantly. The experimental results showed that AF-FD at the concentrations of 25 and 50 μg/mL alle- viated the IFN-� -induced inhibition of MTT reduction by 12.58% and 17.51%, and decreased LDH leakage by 14.47% and 15.83%, respectively. Through FCM and Hoechst 33342 staining, it was found that AF-FD can lower IFN-� induced apoptosis at the concentrations of 12.5-50 μg/mL. The administration of 25 μg/mL AF-FD attenuated the IFN-� induced over-expression of K17 from 206.32% to 178.83%. The effect of AF-FD on K17 expression may play a key role in pro- tecting HaCAT cells from apoptosis.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.