Effect of Th17/Treg imbalance in the peripheral blood of asthma patients

Abstract

Objective To determine the value and effcet of Th17/CD4+ T cells and Treg/CD4+ T cells in the peripheral blood of patients with asthma in acute exacerbation before and after the injection of shenmai intervention. Methods The study select 25 healthy people as the control group, 25 people with acute exacerbation of asthma and 25 people with non-acute exacerbation of asthma.These people were devided into 1640 intervention and the injection of shenmai intervention.Collecting fasting vein blood to determine the ratio of Th17/CD4+ T cells, Treg/CD4+ T cells and the level of IL-10 by flow cytometry. Results ①The level of CD4+ Th17/CD4+ T cells and Th17/CD4+ Treg cells: the acute exacerbation group (3.05±1.27, 1.49±1.78) was higher than the remission group (2.38±0.93, 0.61±0.24) and the control group (1.19±0.39, 0.30±1.14), the differences were statistically significant (P<0.05), the remission group was higher than the control group, the differences were statistically significant (P<0.05). The level of CD4+ Treg/CD4+ T cells: the the control group (5.20±3.26) was higher than the remission group (3.99±0.90) and the acute exacerbation group (2.76±0.93), the differences were statistically significant (P<0.05), the remission group was higher than the acute exacerbation group, the differences were statistically significant (P<0.05). The level of IL-10: the the control group (5.28±1.31) was higher than the remission group (4.46±0.83) and the acute exacerbation group (3.90±0.64), the differences were statistically significant (P<0.05), the remission group was higher than the acute exacerbation group, the differences were statistically significant (P<0.05). ②The level of CD4+ Th17/CD4+ T cells and Th17/CD4+ Treg cells: the pre-treatment was higher than the treated one, the differences were statistically significant (P<0.05). The level of CD4+ Treg/CD4+ T cells: the treated one was higher than the pre-treatment, the differences were statistically significant (P<0.05). The level of IL-10: the treated one was higher than the pre-treatment, the differences were statistically significant (P<0.05). Conclusions The injection of shenmai can adjust this imbalance of Th17/CD4+ Treg cells that exist in the patients with asthma. Key words: Bronchial asthma; Injection of shenmai; Th17 cells; Treg cells