Effect of TGF-β2 on the Mechanical Properties of Posterior Scleral Fibroblasts in Experimental Myopia.

Abstract

Objective The effects of TGF-β2 on mechanical properties of sclerotic desmocytes isolated from healthy and myopic guinea pigs were investigated in order to further understand the pathogenesis of myopia. To study the effect of TGF-β2 on the mechanical properties of posterior scleral fibroblasts in experimental myopia. Methods A lens-induced myopia (LIM) animal model was developed in 12 guinea pigs, with the opposite eye serving as a self-control (SC). Five untreated guinea pigs served as normal controls. Lenses were removed 30 days after model onset. Primary scleral fibroblasts were isolated and passaged twice and then treated with vehicle control or 1, 10, or 100 ng/mL TGF-β2. After 24 h, micropipette aspiration was used to investigate the viscoelastic properties of the cells. Results Scleral fibroblasts from LIM exhibited significantly higher equilibrium moduli and apparent viscosities relative to SC without TGF-β2 treatment. Treatment of LIM or SC scleral fibroblasts with 1 or 10 ng/mL TGF-β2 led to significantly different (p < 0.05) equilibrium moduli and apparent viscosities compared with vehicle control, whereas no significant differences were observed upon treatment with 100 ng/mL TGF-β2. LIM cells treated with 1 and 10 ng/mL TGF-β2 exhibited lower equilibrium moduli and apparent viscosities compared with similarly treated SC cells, but LIM cells and SC cells treated with 100 ng/mL TGF-β2 had similar mechanical properties. Conclusions The addition of 1 and 10 ng/mL TGF-β2 can lower the equilibrium modulus and apparent viscosity of scleral fibroblasts in the normal eye.