Effect of tetramethylpyrazine on adhesion and invasion ability of cell PG in hypoxic environment

Abstract

Objective To investigate the Effect of Tetramethylpyrazine on adhesion and invasion ability of Cell PG in Hypoxic Environment. Methods The revival cell PG was divided into three dosages（high, medium and low）group of Tetramethylpyrazine, DDP group and negative control group. All of five groups were cultured separately in the normal and hypoxic environment. The adhesion ability of Cell PG were detected by MTT. The invasion ability of Cell PG were detected by Transwell chamber. Results Observed on the reconstruction of the basement membrane adhesion in PG cells by MTT, in normoxic environment the mean absorbance of the TMP high, medium and low concentrations, cisplatin group, control group were （0.556±0.040）,（0.519±0.056）, （0.517±0.061）,（0.333±0.057）,（0.300±0.066）, P〈0.01. In hypoxic environment the mean absorbance of the TMP high , medium and low concentrations, cisplatin group, control group were （0.48±0.032）,（0.452±0.059）,（0.274±0.093）,（0.464±0.099）,（0.155±0.042）, P〈0.01. Promotion of cell adhesion with Tetramethylpyrazine of high, medium and low concentration group and DDP group in hypoxic environment is greater than in normoxic environment（P〈0.01）. Observed the reconstruction of the invasion of basement membrane of PG cells by Transwell chamber, in normoxic environment the invasive cells of the TMP high, medium and low concentrations, cisplatin group, control group were ：（28.00±17.00）,（91.67±30.37）,（105.67±39.58）,（85.00±8.19）,（152.33±24.71）,＆amp;nbsp;P<0.05. In hypoxic environment the invasive cells of the TMP high , medium and low concentrations , cisplatin group, control group were （20.33±15.31）, （22.33±17.62）,（56.00±11.53）,（57.67±35.95）, （76.33±18.01）, P<0.05. The invasive cells of normoxic control group was significantly more than hypoxic control group（P<0.05）. Conclusion In hypoxic environment , adhesion and invasion ability of Cell PG significantly decreased compared with normoxic environment ; In normoxia and hypoxia environment, Tetramethylpyrazine and cisplatin could promote the adhesion ability and inhibit the invasion ability of PG cells. Role in promoting of Tetramethylpyrazine on cell adhesion of PG cells was stronger in hypoxic environment. Key words: PG cell; Tetramethylpyrazine; Adhesion; Invasion