Abstract
Injection of testosterone into castrated rats results in increased sequence complexity of nuclear and polysomal RNA in the prostate two hours after the hormone administration as determined by saturation hybridization with unique sequence rat DNA. A small increase in the ratio of complexity of nuclear RNA to polysomal RNA in the androgen-treated prostate has been observed, suggesting possible androgen-mediated post-transcriptional control. However, testosterone treatment induces relatively more complexity in the nuclear RNA populations than that in the polysomal RNA. The results indicate that many of the androgen-stimulated nuclear transcripts are discarded in the nucleus.
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