Abstract
The effect of temperature, salinity and photoperiod on the number of trunk vertebrae in Ambystoma maculatum was studied using field collected eggs from Harrison stages 2-5. Eggs were divided into comparable experimental groups and maintained under controlled conditions. Groups were maintained at different temperatures (5, 10, 15, 20, 25 C), salinities (3 and 6%0, using 1.5%o as a control) and photoperiods (11, 12, 14 hrs light/day). Vertebral counts on 502 cleared and stained animals ranged from 13-15 with a mode of 14 trunk vertebrae. Vertebral number was highest at the lowest temperature at which development will occur. The number of vertebrae increased with increasing salinity, and there was no effect of photoperiod.
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