Abstract
The hydatid materials were collected and studied, so they were contained 50 fertile human hydatid cases {33 (66%) females and 17 (34%) males}. They were collected from Al-Ramadi General Hospital during the period from December, 2003 to July, 2004 .Cysts were observed in 40 (80%) from the liver, 5 (10%) from the lungs, 3 (6%) from the kidney and 2 (4%) cysts from urinary bladder. The specimens were taken from patients of different ages. The in vitro viability of protoscoleces was assessed on the basis of flame cell activity and eosein exclusion, which were considered as criteria to determine the death or viability of protoscoleces. In addition to this movement (flame cell activity), another motility like constriction – relaxation (invagination - evagination) in the protoscoleces was also noticed. Both types of movement were examined under light microscope. The motility of protoscoleces examined under effect of three different temperature degrees 25?C, 37?C, and 40?C was within 15 minutes. It showed steadily increase with rising temperature. Flame cell activity increased as high as 70.01% at 40?C, while the motility with constriction -relaxation movement increased as 100.0% at 40?C. The volumes of hydatid cysts were also studied, so they were measured. Therefore, the volumes of human hydatid cysts were the highest in the lung and this is may be due to the spongy texture of the lung tissues. There is relationship between the volumes of Hydatid cysts and the viability was recorded.
Highlights
Hydatid disease was important various methods for detecting the public health problem [1,2] .It is a fertility and assessing the viability of parasitic infection caused by larval hydatid cysts [9]
Viability of cysts was determined by two methods: 1. Flame cell activity was the activity produced afaint flickering movement [12] by flame propulsion which had led to the use of flickering movement as criterion in assessing the rate of activity .The motility of protoscoleces calculated as flame activity from one point to another and return to the same point
The magnitude of biological activity of protoscoleces in these cases was presented by the proportion of protoscoleces with flame activity or constriction – relaxation movement [10]
Summary
Hydatid disease was important various methods for detecting the public health problem [1,2] .It is a fertility and assessing the viability of parasitic infection caused by larval hydatid cysts [9]. Information relative stage of Echinococcus granulosus to agree of fertility and viability were [3,4]. In vitro ,the viability and motility of the biological cycle of that parasite and protoscoleses with three degrees of it is of particular interest in primary temperature (25oC,37oC and 40oC), and secondary infection [7,8]. There are many studies that used of hydatid cysts and study the relationship between the volumes of hydatid cysts and their viability Some cysts may remain undetectable should be considered in many program for many years until they become large to control and eradicate the disease enough to affect other organs [5,6].It is [10,11].
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