Abstract
Glycation process occurs in protein and becomes more pronounced in diabetes when an increased amount of reducing sugar is present in bloodstream. Glycation of protein may cause conformational changes resulting in the alterations of its binding properties even though they occur at a distance from the binding sites. The changes in protein properties could be related to several pathological consequences such as diabetic and nondiabetic cardiovascular diseases, cataract, renal dysfunction and Alzheimer’s disease. The experiment was designed to test the impact of glycation process on sulfonylurea drug tolbutamide-albumin binding under physiological (T = 309 K) and inflammatory (T = 311 K and T = 313 K) states using fluorescence and UV-VIS spectroscopies. It was found in fluorescence analysis experiments that the modification of serum albumin in tryptophanyl and tyrosyl residues environment may affect the tolbutamide (TB) binding to albumin in subdomain IIA and/or IIIA (Sudlow’s site I and/or II), and also in subdomains IB and IIB. We estimated the binding of tolbutamide to albumin described by a mixed nature of interaction (specific and nonspecific). The association constants (L∙mol−1) for tolbutamide at its high affinity sites on non-glycated albumin were in the range of 1.98–7.88 × 104 L∙mol−1 (λex = 275 nm), 1.20–1.64 × 104 L∙mol−1 (λex = 295 nm) and decreased to 1.24–0.42 × 104 L∙mol−1 at λex = 275 nm (T = 309 K and T = 311 K) and increased to 2.79 × 104 L∙mol−1 at λex = 275 nm (T = 313 K) and to 4.43–6.61 × 104 L∙mol−1 at λex = 295 nm due to the glycation process. Temperature dependence suggests the important role of van der Waals forces and hydrogen bonding in hydrophobic interactions between tolbutamide and both glycated and non-glycated albumin. We concluded that the changes in the environment of TB binding of albumin in subdomain IIA and/or IIIA as well as in subdomains IB and IIB influence on therapeutic effect and therefore the studies of the binding of tolbutamide (in diabetes) to transporting protein under glycation that refers to the modification of a protein are of great importance in pharmacology and biochemistry. This information may lead to the development of more effective drug therapy in people with diabetes.
Highlights
Serum albumin plays a significant role in drugs pharmacokinetics and can affect pharmacological or toxicity effect of the drug
Because the glycation may cause a number of structural changes in the spatial albumin structure that can affect the binding of ligands, the aim of the study was to investigate the influence of glycation that can affect the binding of ligands, the aim of the study was to investigate the influence of glycation process on tolbutamide-albumin binding in temperature corresponding to the human physiological process binding temperature the human physiological (T = 309onK)tolbutamide-albumin and inflammatory states
Our study suggests that glycation of bovine serum serum albumin albumin (BSA) by fructose influences on the tertiary structure of albumin and modifies the fluorescence quenching mechanism of the TB-BSA complex.13 of 20
Summary
Serum albumin plays a significant role in drugs pharmacokinetics and can affect pharmacological or toxicity effect of the drug. Molecules 2017, 22, 569; doi:10.3390/molecules22040569 www.mdpi.com/journal/molecules regions, namely Sudlow’s site I and II which are located within specialized cavities in subdomains regions, namely Sudlow’s [2]. II whichmodification are located within specialized cavities or in pathological subdomains. When protein modification induced by physiological or pathological changes occurs, an alteration of the native conformation and efficiency of major binding sites can be changes occurs, an alteration of the conformation efficiency that of major binding sites can be expected [3]. Albumin is exposed to native numerous structural and modifications can influence its stability, expected [3].physical. Albumin is exposed numerousthus structural modifications that can influence its stability, activity and and chemicaltoproperties, affect the functions performed by this protein [4,5]. Activity and physical and chemical properties, affect the functions performed by this protein.
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