Effect of temperature on theRFof porphine and its metal complexes in normal and reversed-phase TLC

Abstract

Porphine and its complexes with Cu(II), Ni(II), and Zn(II) have been separated by adsorption and partition TLC. In adsorption TLC silica gel was used as stationary phase, with chloroform- n -hexane 5:5, 6:4, and 7:3 ( v/v ) as mobile phases. In partition TLC on RP-18, methanol, methanol-water 9:1 ( v/v ), ethanol, and ethanol-water 9:1 ( v/v ) were used as mobile phases. The objective of the study was determination of the effect of temperature (5 ± 2, 15 ± 2, 25 ± 2, 35 ± 2, 45 ± 2, and 55 ± 2°C) on the retention of the compounds by comparison of the chromatographic separations obtained.