Effect of temperature on the uptake of waterborne strontium in the common carp, Cyprinus carpio (L.)

Abstract

The effect of temperature on the uptake kinetics of strontium (Sr) in the common carp ( Cyprinus carpio) was studied in vivo, exposing pre-acclimated fish to a wide range of Sr concentrations in water (Sr total=0.2–10 000 μM; Ca total=348 μM) at 10, 20, 25 and 30°C. Sr uptake rates were determined in the whole body, gills and blood of the fish after an exposure period of 3 h and were analyzed as a function of the free-ion activity of Sr and Ca in water. The uptake of Sr 2+ by the whole body, gills and blood increased with temperature and showed saturation kinetics with the increase of Sr 2+ activity. Analyzing the observed uptake rates with a Michaelis–Menten type model showed that the kinetic parameters ( J max, K m and K i) for both Sr 2+ and its analogue Ca 2+ are temperature dependent. Thermodynamic analysis of the temperature effects indicates that the Arrhenius activation energies ( E a) required for Sr 2+ uptake (91.9 kJ mol −1) and Ca 2+ uptake (105.9 kJ mol −1) in the whole body of carp were constant over the temperature range 10–25°C and showed a break in the Arrhenius plots above this temperature. The Arrhenius plot for the Sr 2+ uptake in blood was similar to that for the whole body uptake with an E a of 98.1 kJ mol −1. However, the E a for Sr 2+ uptake in gills was much smaller and constant (58.1 kJ mol −1) over the temperature range of 10–30°C. For a temperature change from 10 to 25°C, the Q 10 for Sr 2+ uptake in whole fish, gills and blood were 3.71, 2.29 and 4.05, respectively. Compared with Ca 2+ uptake, Sr 2+ uptake appears to require a lower activation energy for transport across the solution body interface in carp. The similar pattern of Arrhenius plots and magnitude of activation energies for Sr 2+ uptake both in blood and whole fish suggest that the uptake into the blood across the basolateral membrane is the rate-limiting energy barrier and hence dictates the overall uptake rate of Sr 2+ in whole fish.