Abstract
CSVd-infected chrysanthemum plants grown under <TEX>$10^{\circ}C\;or\;15^{\circ}C$</TEX> growth chamber for 2 months resulted a higher dilution endpoint of template RNA for Reverse transcription and polymerase chain reaction (RT-PCR) than those grown for 1 month: <TEX>$10^{-4}(1.35{\times}10^{-2}ug/ml)$</TEX> for 1 month, and <TEX>$10^{-3}(1.35{\times}10^{-1}ug/ml)$</TEX> for 2 months. Independent experiment, shoots cut from CSVd (Chrysanthemum stunt viroid)-in footed chrysanthemum plants grown under <TEX>$10^{\circ}C\;or\;20^{\circ}C$</TEX> growth chamber for 2 months showed the same CSVd concentration as control(<TEX>$30^{\circ}C$</TEX>) at 8 weeks after moving them to normal green-house condition(<TEX>$30^{\circ}C$</TEX>). From those results, it was concluded that even though the concentration of CSVd was reduced in plants grown at low temperatures, when they were moved to normal glass-house temperature CSVd concentration increased to that of untreated plants in 8 weeks. This conclusion was supported by the rapid replication of CSVd in chrysanthemum after infection.
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