Effect of temperature on residual force enhancement in single skeletal muscle fibers

Abstract

It is well accepted that the steady-state isometric force following active stretching of a muscle is greater than the steady-state isometric force obtained in a purely isometric contraction at the same length. This property of skeletal muscle has been called residual force enhancement (FE). Despite decades of research the mechanisms responsible for FE have remained largely unknown. Based on previous studies showing increases in FE in fibers in which cross-bridges were biased towards weakly bound states, we hypothesized that FE might be associated with a stretch-induced facilitation of transitioning from weakly to strongly bound cross-bridges. In order to test this hypothesis, single fibers ( n=11) from the lumbrical muscles of frog ( Rana pipiens) were used to determine FE at temperatures of 7 and 20 °C. At the cold temperature, cross-bridges are biased towards weakly bound states, therefore we expected FE to be greater at 7 °C compared to 20 °C. The average FE was significantly greater at 7 °C (11.5±1.1%) than at 20 °C (7.8±1.0%), as expected. The enhancement of force/stiffness was also significantly greater at the low (13.3±1.4%) compared to the high temperature (5.6±1.7%), indicating an increased conversion from weakly to strongly bound cross-bridges at the low temperature. We conclude from the results of this study that muscle preparations that are biased towards weakly bound cross-bridge states show increased FE for given stretch conditions, thereby supporting the idea that FE might be caused, in part, by a stretch-induced facilitation of the conversion of weakly to strongly bound cross-bridges.