Effect of Temperature on Dissociation of Adult Mouse Liver with Sodium Tetraphenylboron (TPB).

Abstract

Summary1. Dissociation of adult mouse liver in vitro was found to proceed more rapidly at 38° than at 4°C. The number of cells recovered either in the presence or the absence of TPB was increased. The physiological condition of the cells recovered by dissociation at 38°C was superior. 2. At 38°C, the concentration of TPB required for dissociation correlates well with the concentrations where it complexes K+. 3. A procedure has been developed which results in the recovery of about 80% of the total population of parenchymal cells as a suspension of single intact cells.