Effect of temperature and prolonged anoxia exposure on electrophysiological properties of the turtle (Trachemys scripta) heart

Abstract

Cardiac activity of the turtle (Trachemys scripta) is greatly depressed with cold acclimation and anoxia. We examined what electrophysiological modifications accompany and perhaps facilitate this depression of cardiac activity. Turtles were first acclimated to 21 degrees C or 5 degrees C and held under either normoxic or anoxic (6 h at 21 degrees C; 14 days at 5 degrees C) conditions. We then measured cardiac action potentials (APs) using spontaneously contracting whole heart preparations and whole cell current densities of sarcolemmal ion channels using isolated ventricular myocytes under appropriate normoxic and anoxic conditions. Compared with 21 degrees C-acclimated turtles, 5 degrees C-acclimated turtles exhibited a less negative resting membrane potential (by 18-29 mV), a 4.7- to 6.8-fold slower AP upstroke rate, and a 4.2- to 4.9-fold greater AP duration. Correspondingly, peak densities of ventricular voltage-gated Na(+) (I(Na)) and L-type Ca(2+) currents and inward slope conductances of inward rectifier K(+) (I(K1)) channel current were approximately 1/7th (Q(10) = 3.4), 1/13th (Q(10) = 5.0), and one-half (Q(10) = 1.4) of those of 21 degrees C-acclimated ventricular myocytes, respectively. With anoxia at 21 degrees C, peak I(Na) density doubled and ventricular AP duration increased by 47%, a change proportional to the reported approximately 30% reduction of intrinsic heart rate. In contrast, with anoxia at 5 degrees C, ventricular AP characteristics were unaffected; of the ion currents investigated, only the inward conductance via I(K1) changed significantly (reduced by 46%). The present findings indicate that cold temperature, more so than prolonged anoxia, results in substantial modifications of cardiac APs and reduction of ventricular ion current densities. These changes likely prepare cardiac muscle for winter anoxia conditions.