Abstract
The effect of temperature on T cell mitogen and mixed lymphocyte responses (MLR) of chicken lymphoid cells in vitro was examined. Responses at 40°C were much higher than at 37°C. This difference did not appear to be due only to faster kinetics of the responses at 40°C. At the lower temperature the MLR could be enhanced by polyethylene glycol (PEG) for spleen cells and by Con A induced lymphokines for peripheral blood cells (PBL). The positive effect of PEG on the chicken spleen cell MLR appeared to be determined at the stimulator cell level. Responses to mitogens at 37°C of both spleen cells and PBL were enhanced by lymphokines.
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