Abstract

This experiment was conducted to evaluate the effect of germination media and three temperatures on five male strains of pistachio at research center of agriculture faculty – Aleppo,(Arab center),.In this experiment, the rates of pollen germination were tested in different germination media (sucrose 10%, sucrose 10%+0.25 gr. boric acid P L. and sucrose 10%+0.0625 gr. boric acid P L.) with 3 temperature levels( 10,20 and 30◦c) .The media was contain 3 gr. agar per liter of water. The results showed that male strain 36 was gave the best germination rate, (78.33 % and tube length 796.1micron) as compared with other strains ,and male strain 47 was the lower (63.11%,511.3 micron) tube germination and tube length (63.11%,511.3 micron) respectively, but all were gave high rates of germination. sucrose 10% media gave the best germination rate (77.07%) and sucrose 10%+ 0.25 gr. boric acid P L. gave the lowest germination rate (62.2%), sucrose 10%+0. 0.25 gr. boric acid P L. gave highest tube germination length (777.68micron), where as 10% sucrose +0.0 625gr. boric acid P L. was the lowest tube length (499.88micron) .On the other hand, 30◦c gave the best germination rate and pollen tube germination length (88.73%and 1005.26 micron), however 10◦c gave lowest rates of these parameters (45.53% and, 244.3 micron) for germination rate and tube pollen length respectively. Generally, all media were gave highest significant results in germination rates and pollen tube length for all strains at all temperatures. we conclude that sucrose 10%was better media for pollen germination and sucrose 10%+0. 25 gr. boric acid P L.was the best for pollen tube length .

Highlights

  • Pistachio trees are dioecious with male and female flowers on separate trees

  • Acar and AK (2007) Reported that the highest germination rate of pistachio pollen was obtained from sucrose 20% (87.78%),and they found that adding H3BO3 20 and 70 ppm germ ination rate obtained over 80%.Ulkumen (1945) reported that the highest germination rate was obtained from 10 and 15% sucrose solution in Pistacia vera, P. terebinthus, P. atlantica

  • MATERIAL AND METHODS In this experiment 5 male strains of pistachio(36, 38, 42, 47, and57) grown in pistachio orchard at Arab center farm in Aleppo were used as material.Pollen collection: The branches of selected male types were taken to the laboratory conditions (20 c) before the cluster dehisced pollen, and they were put in glass container filled with water

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Summary

Introduction

Pistachio trees are dioecious with male and female flowers on separate trees. Male and female trees must be present for cross pollination and fruit set. In the Arab Center Farm for studies of Arid Zone and dry lands at Aleppo ,there are a lot of different male strains used as a pollinators of pistachio trees. It is necessary to know a viability and germination rate of pollen grain before pollination date to be able to ensure fruit set(Dafni&Firmage,2000) .There are many studies published about germination media and temperature in recent years .Sfendiaro et al, (2006) reported that pollen viability in TTC test was ranged between 85.7 and 98.85% in different male types of pistachio. Hadj-Hassan (1985) was arranged male types of pistachio according to germination rate from weak to excellent germination Therios et al(1985) showed that maximum germination obtained at 35,20 and 25%sucrose for the clones A, B and C of pistachio male strains respectively

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