Abstract
Abstract Research was conducted to determine the effect of temperature and fermentation time of crude cellulase production by Trichoderma reesei on straw substrate. The research was arranged in a randomized complete factorial design consisting of two factors. The first factor is the temperature of fermentation consists of two levels, 27 C and 37 C. The second factor is the time fermentation consists of three levels, namely 5 d, 7 d, and 9 d. The results showed that the temperature and fermentation period significantly affect the observed parameters on crude cellulase production by Trichoderma reesei . The optimal treatment combination to produce crude cellulase with maximum activity is at 27 C and fermentation time of 9 d with an average value of cellulase activity (filter paperase) and soluble protein respectively 0.041 193 7 unit·mL –1 and 0.021 613 mg·mL –1 .
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