Abstract
Testicular testosterone was determined by radioimmunoassay in the frog ( Rana esculenta) kept in total darkness, at a high or a low temperature (24 or 4°C), and treated with a gonadotrophin-releasing hormone analog (GnRHa, HOE 766). Prolonged exposure to dark conditions seemed to inhibit hypotalamic functions. Moreover, it is shown that high temperature interacts positively with GnRHa treatment on testicular testosterone concentration.
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