Abstract
Tea polyphenols are known to play roles in critical steps of human lung carcinoma cell metastasis. For understanding the mechanisms whereby they inhibit tumor metastasis, the present study was conducted to investigate their effects on the adhesion of highly metastatic lung carcinoma cell lines (PG cells) to endothelial cells (EC cells) and adhesion molecule expression in vitro. The expression of CD44 or CD54 in the PG cells was detected by flow cytometry and adhesion of PG cells to EC cells was assessed by confocal microscopy double fluorescence staining. The results showed that tea polyphenols: (1) inhibited the expression of CD44 and CD54, two important adhesion molecules in the PG cells in a dose-dependent manner; (2) significantly blocked the adhesion of PG cells to EC cells not only in a state of rest but also when active; and (3) influenced CD44 and CD54 expression during the adhesion process of PG cells to EC cells. The data indicated that the blocking role of tea polyphenols in the adhesion of PG cells to EC cells is related to CD44 and CD54. The mechanism of tea polyphenol prevention of human lung carcinoma metastasis might be through inhibiting adhesion molecule expression to block cancer cell adhesion.
Highlights
Tumor hematogenous metastasis is a complex multistep pathological process
The results showed that tea polyphenols: (1) inhibited the expression of CD44 and CD54, two important adhesion molecules in the PG cells in a dose-dependent manner; (2) significantly blocked the adhesion of PG cells to EC cells in a state of rest and when active; and (3) influenced CD44 and CD54 expression during the adhesion process of PG cells to EC cells
The data illustrated that tea polyphenols could inhibit CD44 and CD54 expression on the PG cells in a dose-dependent manner
Summary
Tumor hematogenous metastasis is a complex multistep pathological process. One crucial step is the mutual adhesion and interaction between cancer cells and vascular endothelial cells (Orr et al, 2000; Guo et al, 2007). Known as catechins, account for 30–42% of the dry weight of the solids in brewed green tea (Mukhtar et al, 1999). They have effects on cancer prevention, inhibition and anti-metastasis (Liang et al, 1999; Lin et al, 1999). Few researches have been reported about inhibitory effects of tea polyphenols on hematogenous metastasis of lung cancer cells. The object of the present study was to investigate the mechanism of hematogenous metastasis of lung cancer cells and the anti-metastatic effects of tea polyphenols
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