Effect of taurocholic acid and BSP on conjugation patterns and suspension stability of human and rat bile

Abstract

Experiments were carried out to demonstrate conjugation patterns in rat bile during the formation and dissolution of lithocholic acid stones. Stone dissolution was clearly related to increased excretion of taurine conjugates and reduction of the glycine to taurine conjugation ratio. Man has a similar dual conjugating mechanism for bile acids, and feeding of 3.0 gm of taurocholic acid daily produced increased bile salt excretion and alteration of the glycine to taurine ratio toward taurine conjugation. Acute doses of BSP produced increased bile stability as measured by the increase in negative zeta potential of a standard particle in bile, but interfered with taurine-conjugated and total bile salt excretion at high BSP excretion levels. Use of negatively charged ion excretion in bile, however, represents a fruitful field for further investigation.