Abstract
Twelve practical diets were formulated to contain four levels of Bacillus licheniformis (0.0, 0.24×106, 0.48×106 and 0.96×106CFUg−1), respectively, with three yeast extract levels (0%, 0.5% and 1%), respectively. Each diet was randomly assigned to duplicate groups of 50 Nile tilapia (Oreochromis niloticus) (5.99±0.03g) in 24 concrete ponds (0.5m3 and 1.25m depth) for 12weeks. Increasing dietary B. licheniformis levels in O. niloticus and yeast extract levels significantly (P<0.01) improved growth performance and nutrient utilization. Supplementation of the experimental diets with, 0.48×106CFU/g−1 and 1.0% yeast extract showed the best nutrient utilization compared to other treatments. All probiotic levels significantly (P<0.01) increased chemical composition (P<0.05) compared to the control group, while increasing yeast extract did not significantly alter chemical composition. Hematological indices, total protein and albumin of O. niloticus significantly increased while aspartate aminotransferase and alanine aminotransferase significantly (P<0.01) decreased with an increase in B. licheniformis level up to 0.48×106CFUg−1. Increasing levels of yeast extract had no effect on hematological parameters and the diets supplemented with 0.48×106CFUg−1 and 0.5% yeast extract showed the highest hematological values.
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