Abstract
This study was conducted to investigate the effect of swim-up and glass wool as sperm separation techniques with adding vitamin E and superoxide dismutase (SOD) to Tris extender on improving post-cryopreserved total sperm characteristics in straw and freezability percentage for low semen quality of Holstein bulls. Low and good of semen were extended using Tris extender. Good semen quality (GSQ) was divided into 3 groups (L1; Tris extender, L2; 2 mM vitamin E, L3; 200 IU SOD) Low semen quality (LSQ) was divided into two main groups, and subdivided into 3 sub-groups (L4; Tris extender, L5; 2 mM vitamin E, L6; 200 IU SOD). In the second main group, swim-up and glass wool techniques were used with adding vitamin E and SOD and subdivided into 3 sub-groups with each technique, and referred to L7, L8 and L9 for swim-up technique and L10, L11 and L12 for glass wool technique. Sperms with good motile intact acrosome and plasma membrane normal morphology of sperm were obtained using the glass wool separation technique with adding vitamin E.
Highlights
The enhancement in quality of semen is crucial aspect for maximum utilization of genetically-superior sub-fertile sire, for the reason of age and/or non-specific factors like thermal stress, transport and vaccination stress during their dynamic life show a low-grade semen quality than previously (25)
Swim-up technique is based on migration of motile sperm from the bottom to the top fraction of the media, which separates motile sperms from the non-motile sperm and debris, as it is based on the ability of sperm to swim into the culture medium
This study was undertaken to explore the effect of using swim-up and glass wool techniques with adding vitamin E or superoxide dismutase (SOD) to separated, low and good-quality semen on improving postcryopreserved totals sperm normal morphology counts, total acrosome integrity count,total plasma membrane integrity, total function of sperm fraction and freezability percentage pertaining to low semen quality of Holstein bulls
Summary
The enhancement in quality of semen is crucial aspect for maximum utilization of genetically-superior sub-fertile sire, for the reason of age and/or non-specific factors like thermal stress, transport and vaccination stress during their dynamic life show a low-grade semen quality than previously (25). This study was undertaken to explore the effect of using swim-up and glass wool techniques with adding vitamin E or SOD to separated, low and good-quality semen on improving postcryopreserved totals sperm normal morphology counts, total acrosome integrity count ,total plasma membrane integrity, total function of sperm fraction and freezability percentage pertaining to low semen quality of Holstein bulls. This study was aimed the effect of using swim-up and glass wool techniques with addition of vitamin E or SOD to separated, low and good-quality semen on PC sperm characteristics of Holstein bulls. PC exhibited greater (P≤0.03) total normal morphology counts (×106) as compared with 3rd and 6th months PC period in L3 and L6 groups (Table 1). Non- significant differences were noticed in the total normal morphology counts (×106) at 3rd and 6th months PC period in L3 and L6 groups (Table 1). Non-significant differences were demonstrated in the total normal morphology counts (×106) at 3rd as compared with 96 hrs. and 6th months higher (P≤0.03) total normal morphology PC period in L3 and L6 groups (Table 1)
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