Abstract
The herbicide SW26 and erythrosin B (EB) both inhibited ATPase activity in microsomal and plasma membrane-enriched fractions isolated from Ricinus cotyledons and cucumber hypocotyls. Both inhibitors were more effective with the vanadate-sensitive plasma membrane fraction. With intact tissues, EB was an effective inhibitor of both medium acidification and sucrose uptake by Ricinus cotyledons, whereas SW26 had no effect. EB was also a more effective inhihitor than SW26 of medium acidification and elongation growth by cucumber hypocotyls. Neither EB or SW26 affected tissue respiration. Thus, these reagents are useful additions to the limited range of inhibitors available to investigate the function of proton-pumping ATPases using both isolated membrane fractions and intact tissues.
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