Effect of supplementing an α-amylase enzyme or a blend of essential oil components on the performance, nutrient digestibility and nitrogen balance of dairy cows

Abstract

Lowering the dietary protein content is a promising strategy to reduce N excretions in cattle but requires an improved N utilization by the animal. Feed enzymes (e.g., exogenous α-amylase) and plant extracts (e.g., essential oils (EO)) are 2 additives which may enhance rumen function and possibly also microbial protein yield. This may increase fat and protein corrected milk yield (MY) and milk nitrogen efficiency (MNE) and thus lower N losses from dairy cows. Both types of additives were studied in an experiment including 39 Holstein cows (average ± SD: 40.7 ± 7.95 kg/d MY, 89 ± 43 d in milk (DIM), 2.7 ± 1.5 lactations, 677 ± 68.6 kg of BW) consisting of a covariate (4 weeks) and treatment period (5 weeks). During the whole experiment cows were fed a typical Benelux diet (CTRL), supplemented with concentrates to meet individual requirements for energy and metabolizable protein, which were fulfilled for 100% and 101%, respectively. The total diet was low in crude protein (15.5%) and relatively high in starch (22.6% and 6.6% rumen bypass starch). Cows were balanced for parity, DIM, MY and roughage intake and randomly assigned to one of 3 groups, receiving the following treatments in the treatment period; (1) CTRL (n = 13); (2) CTRL + 14 g/cow/d Ronozyme RumiStar (α-amylase enzyme, DSM) (AMEZ, n = 13); (3) CTRL + 2.5 g/cow/d Crina Protect (blend of EO components, DSM) (ESOL, n = 13). Animal performance, ruminal pH and enteric gas emissions were monitored throughout the experiment. During the last week of the covariate and treatment period, nitrogen balances were conducted, total-tract nutrient digestibility was determined and urinary allantoin and uric acid were determined as indicators for microbial N production. The statistical model applied to these variables contained group and DIM during treatment period as fixed effects and the values from the covariate period as covariate. Post-hoc Dunnet corrected comparisons between each treatment group and the control group were explored. The α-amylase enzyme tended to increase apparent total-tract starch digestibility and increased milk lactose concentration. The EO blend tended to increase milk yield and increased milk N output, MNE and feed efficiency. Therefore, when feeding reduced dietary protein levels, EO have potential to improve the N-use efficiency in cattle, whereas the α-amylase enzyme might increase starch digestibility and milk lactose. However, additional research is necessary to substantiate our findings.