Effect of Supplemented Alcoholic Extract of Annatto (Bixa orellara) Seeds and α-Tocotrienol to Soy-Bean Lecithin Extender on Some Biomarkers of Cryopreserved Semen of Holstein Bulls

Abstract

The purpose of this research was to examine the impact of Soy-bean lecithin extender combined with different quantities of alcoholic extract of annatto (Bixa orellara) and - tocotrienol on sperm abnormalities and certain biomarkers of Holstein bulls semen after being frozen for varying lengths of time. In the experiment, bull semen was pooled, diluted with Soy-bean lecithin extender, and distributed randomly among five groups: Diluted semen was put through the semen cryopreservation technique; the control treatment (C) consisted solely of soy-bean lecithin extender; T1 included 150mg annatto extract/25 ml; T2 contained 200mg annatto extract/25 ml; T3 contained 3mM tocotreinol; and T4 contained 4mM tocotreinol. After 48 hours, 3 months, and 6 months, both chilled and frozen semen were evaluated for abnormalities, Malondialdehyde (MDA), Total antioxidant capacity (TAC) concentration, and DNA fragmentation percentage. As shown by the data, there were no significant differences between the treatments in terms of total abnormalities in sperm after any of the cryopreservation times, but there were significant differences in terms of MDA and DNA fragmentation. Some of the biomarkers in Holstein bulls’ sperm were found to be significantly improved when a combination of alcoholic extract of annatto(Bixa orellara) and tocotrienol was added to Soy-bean lecithin.