Effect of supplementation of yeast with bacteriocin and Lactobacillus culture on growth performance, cecal fermentation, microbiota composition, and blood characteristics in broiler chickens.

C. Y. Chen,H. T. Wang,S. W. Chen

doi:10.5713/ajas.16.0203

Abstract

ObjectiveThe aim of the present study was to investigate the effect of yeast with bacteriocin and Lactobacillus cultures (mixture of Lactobacillus agilis BCRC 10436 and Lactobacillus reuteri BCRC 17476) supplements, alone or in combination, on broiler chicken performance.MethodsA total of 300, 1-d-old healthy broiler chickens were randomly divided into five treatment groups: i) basal diet (control), ii) basal diet+0.25% yeast (Saccharomyces cerevisiae) (YC), iii) basal diet+0.25% yeast with bacteriocin (BA), iv) basal diet+Lactobacillus cultures (LAB), and v) basal diet +0.25% yeast with bacteriocin+Lactobacillus cultures (BA+LAB). Growth performance, cecal microbiota, cecal fermentation products, and blood biochemistry parameters were determined when chickens were 21 and 35 d old.ResultsThe supplementation of YC, BA, and BA+LAB resulted in a significantly better feed conversion rate (FCR) than that of the control group during 1 to 21 d (p<0.05). The LAB supplementation had a significant effect on the presence of Lactobacillus in the ceca at 35 d. None of the supplements had an effect on relative numbers of L. agilis and L. reuter at 21 d, but the BA supplementation resulted in the decrease of both Lactobacillus strains at 35 d. The BA+LAB supplementation resulted in higher short chain fatty acid (SCFA) in the ceca, but LAB supplementation significantly decreased the SCFA at 35 d (p<0.05). All treatments tended to decrease ammonia concentration in the ceca at 21 d, especially in the LAB treatment group. The BA supplementation alone decreased the triacylglycerol (TG) concentration significantly at 21 d (p<0.05), but the synergistic effect of BA and LAB supplementation was required to reduce the TG concentration at 35 d. The YC supplementation tended to increase the plasma cholesterol at 21 d and 35 d. However, the BA supplementation significantly decreased the cholesterol and low density lipoprotein cholesterol level at 35 d. In conclusion, the BA+LAB supplementation was beneficial to body weight gain and FCR of broiler chickens.ConclusionThe effect of BA and LAB supplementation may be a result of the growth of lactic acid bacteria enhancement and physiological characterization of bacteriocin, and it suggests that the BA and LAB supplementation level or Lactobacillus strain selection should be integrated in future supplementation designs.

Highlights

Antibiotics have been widely used as feed additives for broiler chickens, owing to their ability to manipulate the intestinal microbial population, improve feed conversion rate (FCR), and promote growth
Patterson and Burkholder [1] indicated that animal efficacy for most probiotics could be supplemented with an intake of about 108 to 109 CFU/d, but over-supplementation had no benefit on growth performance or FCR
LAB was supplemented in an effective range (107 CFU per gram diet) according to the LAB supplementation concentration and feed intake of broiler chickens in previous studies [13,15,16]

Summary

Introduction

Antibiotics have been widely used as feed additives for broiler chickens, owing to their ability to manipulate the intestinal microbial population, improve feed conversion rate (FCR), and promote growth. A previous study suggested that yeast with albusin B inhibits pathogenic bacteria, increases intestinal absorption of protein and glucose, and improves gut barrier function. It modulated the gut microbiota by increasing cecal Lactobacillus counts and decreasing pathogenic populations [9]. Results of the effect of feeding with Lactobacillus cultures and zinc bacitracin alone, or in combination, on the growth of broiler chickens suggested that food conversion was reduced by zinc bacitracin alone but was improved by the combined use of Lactobacillus cultures and bacitracin [10] In spite of these reports, there is limited information on the function and benefit of the combined supplementation of Lactobacillus cultures and bacteriocin product. The present study was conducted to investigate the effect of sup-

Methods

Results

Conclusion