Abstract
Arthrospira platensis was used to obtain functional extracts through supercritical carbon dioxide extraction (SFE-CO2). Pressure (P), temperature (T), co-solvent (CX), static extraction (SX), dispersant (Di) and dynamic extraction (DX) were evaluated as process parameters through a Plackett–Burman design. The maximum extract yield obtained was 7.48 ± 0.15% w/w. The maximum contents of bioactive metabolites in extracts were 0.69 ± 0.09 µg/g of riboflavin, 5.49 ± 0.10 µg/g of α-tocopherol, 524.46 ± 0.10 µg/g of β-carotene, 1.44 ± 0.10 µg/g of lutein and 32.11 ± 0.12 mg/g of fatty acids with 39.38% of palmitic acid, 20.63% of linoleic acid and 30.27% of γ-linolenic acid. A. platensis extracts had an antioxidant activity of 76.47 ± 0.71 µg GAE/g by Folin–Ciocalteu assay, 0.52 ± 0.02, 0.40 ± 0.01 and 1.47 ± 0.02 µmol TE/g by DPPH, FRAP and TEAC assays, respectively. These extracts showed antimicrobial activity against Staphylococcus aureus ATCC 25923, Pseudomonas aeruginosa ATCC 27853, Escherichia coli ATCC 25922 and Candida albicans ATCC 10231. Overall, co-solvent was the most significant factor for all measured effects (p < 0.05). Arthrospira platensis represents a sustainable source of bioactive compounds through SFE using the following extraction parameters P: 450 bar, CX: 11 g/min, SX: 15 min, DX: 25 min, T: 60 °C and Di: 35 g.
Highlights
In the last few years, we have seen a growing tendency to incorporate bioactive compounds from algae into traditional foods, cosmetics, and pharmaceutical products to replace components obtained by chemical synthesis [1]
The treatments were chosen to cover a range of conditions considering the experimental limitations of the pilot plant scale, analytical equipment, and previous data reported for the extraction of bioactive metabolites from A. platensis [9,10,18,19,24,25,26]
CX plays an important role in the extractions of all the metabolites analyzed, and our results demonstrated that the addition of ethanol enhances the performance of the extraction process
Summary
In the last few years, we have seen a growing tendency to incorporate bioactive compounds from algae into traditional foods, cosmetics, and pharmaceutical products to replace components obtained by chemical synthesis [1]. Mar. Drugs 2017, 15, 174 grows extensively in tropical and subtropical water bodies, characterized by high levels of carbonate and bicarbonate. Drugs 2017, 15, 174 grows extensively in tropical and subtropical water bodies, characterized by high levels of carbonate and bicarbonate These cyanobacteria have been used since ancient times as a source of food, due to their high content of proteins (up to 70% dry weight), amino acids, fatty acids, vitamins, minerals, carbohydrates, phenolic compounds and pigments such as β-carotene and phycocyanins [2,3]. Other studies reported immuno-promoting effects due to increasing in the production of Interferon-γ [6,7], antimicrobial activity against Staphylococcus aureus, Bacillus cereus, Escherichia coli, and Yersinia enterocolitica from its extracts [8], and several pigments (e.g., β-carotene, zeaxanthin, chlorophyll and phycocyanin) that are related to its antioxidant activity [9,10]
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
