Effect of sulodexide on lipopolysaccharide induced human peritoneal mesothelial cells inflammation and fibrosis

Abstract

Objective To explore the expression of glycocalyx (HA and DCN), inflammatory and fibrosis factors(IL-6 and TBF-β1) on human peritoneal mesothelial cells(HPMCs) stimulated by lipopolysaccharide. Methods HPMCs were treated on the following conditions: control group and LPS group. ①LPS stimulated HPMCs for different concentrations: 1-100 μg/mL. ②10 μg/mL LPS stimulated HPMCs for different times: 0-72 h. ③HPMCs were treated with 10 μg/mL LPS and SLX(50-1200 μg/mL) for 48 h. Results Compared to control group, LPS up-regulated the protein of HA, DCN, IL-6 and TGF-β1 with significant difference. Compared to LPS group, treatment of SLX further increased the protein of HA and DCN, down-regulated the expression of IL-6 and TGF-β1. Conclusions LPS up-regulates the protein of glycocalyx, and treatment of SLX repairs glycocalyx through adding GAGs. LPS up-regulates the protein level of inflammatory and fibrosis factors, however, SLX can protect the peritoneal function by inhibiting peritoneal inflammation and fibrosis. Key words: Peritoneal Dialysis; Inflammation; Fibrosis; Peritoneum; Lipopolysaccharide