Effect of sulfated galactans from red seaweed Gracilaria fisheri on extracellular matrix production in human dermal fibroblast

Abstract

Fibroblasts are derived from mesenchymal stem cells that play an important role in produce the extracellular matrix (ECM). ECM creates a tissue microenvironment for regulation of cell signaling. The loss of ECM plasticity is associated with several pathologies, especially those involving chronic inflammation, therefore, the ECM represents a potential therapeutic target for certain conditions. Sulfated galactans was extracted from red seaweed Gracilaria fisheri that previous studies have been reported about its activities on anti‐oxidant, anti‐tumour, anti‐melanogenesis, and anti‐bacteria. Therefore, the aim of this study was to investigate the effect of potential of sulfated galactans from red seaweed G. fisheri on enhancing the ECM production. Cytotoxicity was tested following determination of ECM‐related gene and protein expression on human dermal fibroblast (HDF) by quantitative RT‐PCR and enzyme‐linked immunosorbent assay (ELISA), respectively. The result showed that sulfated galactans not effects to survival rate of HDF. Additionally, we found that sulfated galactans was able to modulate ECM production by regulating the collagen, elastin, hyaluronic acid, MMP‐1, and TIMP production. The present results demonstrating the effects of a natural multi‐component compound on ECM composition, highlighted the possibility of pharmacologically modulating ECM molecules. The recovery and the maintenance of ECM homeostasis might be considered as a potential therapeutic goal to ameliorate pathological conditions.Support or Funding InformationThis work was financially supported by Navamindradhiraj University Research Fund.