Abstract
Effect of sulfate and nitrate on anaerobic degradation of volatile fatty acids at 20°C was investigated. Acetate degradation was found to be independent of the presence of sulfate. The addition of sulfate enhanced propionate/butyrate/valerate oxidation with acetate accumulation. Sulfate appreciably inhibited 14CH4 production from H2+14CO2 only in the absence of an exogenous electron donor. The sulfate-reducing bacterial counts on propionate increased 10-fold in the presence of sulfate compared to control. Whereas, molybdate and nitrate decreased the volatile fatty acids degradation and methane production by inhibiting both SRB and methanogens.
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