Effect of Substrate Sterilization and Spawning Method on the Yield and Yield Attributes of Milky White Mushroom (Calocybe indica)

Md Ferdaus Ahmed,Md Jahedur Rahman,Md Nazrul Islam,Md Jafar Ullah,Nirod Chandra Sarker

doi:10.13189/ujps.2020.080201

Abstract

Sterilization of uncomposted substrate is necessary to avoid contamination and promote mycelium growth for getting maximum yield of mushroom. Spawning method is also important for evenly growth of mycelium in the substrate. Therefore, the present experiment was carried out to investigate the impact of substrate sterilization technique and spawning method on yield and yield attributes of C. indica. Seven different treatments such as Steam treatment of substrate and spawning in 3 layers (T1), Steam treatment of substrate and spawning thoroughly (T2), Autoclaving of substrate and top spawning (T3), Autoclaving of substrate and spawning in 3 layers (T4), Autoclaving of substrate and spawning thoroughly (T5), Hot water treatment of substrate and spawning in 3 layers (T6) and Hot water treatment of substrate and spawning thoroughly (T7) were used in this experiment. Mycelium colonization, substrate contamination, days to total harvest, yield and biological efficiency was significantly affected by substrate sterilization technique and spawning method. Faster mycelium colonization, no substrate contamination, highest yield and biological efficiency was recorded in substrate treated with hot water and inoculated in three layers (T6). Result revealed that hot water treatment in combination with three layer spawning of rice straw substrate was the best substrate sterilization technique & spawning method for milky white mushroom cultivation.

Highlights

Calocybe indica is a tropical edible mushroom of Indian origin and can be cultivated indoor in high temperature and humidity areas (Purkayastha & Chandra, 1974)
Seven different treatments of substrate sterilization technique in combination with spawning methods namely T1= Steam treatment of substrate and spawning in 3 layers, T2= Steam treatment of substrate and spawning thoroughly, T3=Autoclaving of substrate and top spawning, T4= Autoclaving of substrate and spawning in 3 layers, T5= Autoclaving of substrate and spawning thoroughly, T6= Hot water treatment of substrate and spawning in 3 layers and T7= Hot water treatment of substrate and spawning thoroughly were used in this experiment
Mycelium colonization was faster (16.50 days) in substrate treated with hot water and inoculated by thorough spawning (T7) which was statistically similar (18.25 days) to substrate treated with hot water and inoculated in three layers (T6)

Summary

Introduction

Calocybe indica is a tropical edible mushroom of Indian origin and can be cultivated indoor in high temperature and humidity areas (Purkayastha & Chandra, 1974). A substrate is an important substance for making spawn and growing mushrooms (Singh et al 2017). Milky white mushroom can be cultivated on varieties of cellulosic substrates like, paddy straw, wheat straw, maize stalks, sorghum stalks, pearl millet stalks, sugarcane trace, sugarcane baggase, soya bean straw, cotton waste, coconut coir pith, groundnut haulms etc. Paddy straw is the principal substrate used for its cultivation in Bangladesh. K. (2010) stated that paddy straw was the best among the substrates as it produced the maximum yield and biological efficiency (71.2% BE) of C. indica

Methods

Findings

Discussion

Conclusion