Abstract
The influence of substrate loading rate on fermentative hydrogen (H2) production was studied in biofilm configured sequencing batch reactor using chemical wastewater as substrate. Reactor was operated with selectively enriched anaerobic mixed microflora at different organic loading rates (OLRs; 6.3, 7.1 and 7.9kg COD/m3day) after adjusting the feed to a pH of 6.0 (acidophilic) to provide suitable environment for acidogenic bacterial function. Variation in H2 production rate was observed with change in OLR [specific hydrogen yield – 13.44molH2/kgCODRday (6.3kgCOD/m3day), 8.23molH2/kgCODRday (7.1kgCOD/m3day) and 6.064molH2/kgCODRday (7.9kgCOD/m3day)]. H2 yield showed reasonably good correlation with pH drop [6.3kgCOD/m3day (R2 – 0.9796), 7.1kgCOD/m3day (R2 – 0.9973), 7.9kgCOD/m3day (R2 – 0.9908)]. Increase in OLR showed marked reduction in COD removal efficiency [22.6% – 6.3kgCOD/m3day; 19.8% – 7.1kgCOD/m3day and 17.2% – 7.9kgCOD/m3day].
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