Effect of strigolactone on growth, photosynthetic efficiency, antioxidant activity, and osmolytes accumulation in different maize (Zea mays L.) hybrids grown under drought stress

Abstract

ABSTRACT Drought alters plant physiology, morphology, and biochemical pathways, necessitating effective mitigation strategies. Strigolactones (SLs) are phytohormones known to enhance plant growth under abiotic stress. However, their specific impact on drought stress in maize remains unclear. This study aimed to determine the optimal SL concentration for mitigating drought stress in two maize hybrids (HY-1898, FH-1046). Maize plants were subjected to 60% field capacity drought stress in a pot experiment. After 40 d, different concentrations (0, 0.001, 0.01, and 0.1 mg L−1) of the synthetic SL analogue GR24 were applied to evaluate their effects on growth features, photosynthesis attributes, and osmolyte accumulation in the maize hybrids. Results showed that exogenous SL application significantly increased photosynthetic pigments in maize hybrids under drought stress. Chlorophyll content, gas exchange characteristics, net CO2 assimilation rate, stomatal conductance, water use efficiency, and antioxidant activities were enhanced by GR24. Leaf ascorbic acid and total phenolics also increased with SL application. Organic osmolytes, such as glycine betaine and free proline, were elevated in both maize hybrids under drought stress. Yield-related parameters, including cob diameter, cob weight, number of seeds per cob, and number of seeds per plant, were significantly increased by GR24 under drought stress. Our findings highlight the potential of GR24 foliar application to mitigate drought stress and promote maize growth and grain yield in a concentration-dependent manner. The minimum effective SL concentration against drought stress was determined to be 0.01 mg L−1. Overall, foliar application of GR24 could serve as a sustainable approach for drought tolerance in agriculture.