Abstract
Holstein calves given three consecutive i.m. injections of dexamethasone (DEX) (0.04 mg kg −1 showed lymphopenia and neutrophilia with increased numbers of mature neutrophils on post-injection Days 1 and 2, but these values returned to normal levels by post-injection Day 3. Interleukin-2 receptor (IL-2R) expression by peripheral blood mononuclear cells (PBMC) was evaluated by flow cytometry using a monoclonal antibody specific for bovine IL-2Rα. Treatment with DEX significantly decreased expression of IL-2Rα in Concanavalin A (Con A)-activated PBMC on Day 1 ( P < 0.02) and on Day 2 ( P < 0.1). On Day 3, expression of IL-2Rα by PBMC was similar in control and DEX-treated calves. This decrease in IL-2Rα expression correlated with decreased proliferative responses of PBMC to the T-cell mitogens, phytohemagglutinin (PHA) and Con A. Following in vitro treatment with recombinant human (rhu) interleukin-12 (IL-12) Con A-induced proliferative responses of PBMC tended to be higher in both groups. However, the rhu IL-12 induced increases of Con A activated proliferative responses were significantly greater in DEX-treated calves than in control calves. IL-2Rα expression by PBMC was found to be less in calves transported 800 km in a truck as compared to that in PBMC from controls. These data suggest that stress-induced immunosuppression in calves may involve decreased IL-2Rα expression and decreased IL-12 production. Serum chemistry results indicated a trend toward higher creatine kinase (CK) levels in DEX-treated calves. This may be due to the lysis of corticosteroid sensitive lymphocytes.
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